Investigation of a Novel Predictive Biomarker Profile for the Outcome of Periodontal Treatment

• Autores: Sarhang S. Gul, Gareth S. Griffiths, Graham P. Stafford, Mohammed I. Al-Zubidi, Andrew Rawlinson, Charles W.I. Douglas
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 88, Nº. 11, 2017, págs. 1135-1144
• Idioma: inglés
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• Resumen

  • Background: An ability to predict the response to conventional non-surgical treatment of a periodontal site would be advantageous. However, biomarkers or tests devised to achieve this have lacked sensitivity. The aim of this study is to assess the ability of a novel combination of biomarkers to predict treatment outcome of patients with chronic periodontitis.

    Methods: Gingival crevicular fluid (GCF) and subgingival plaque were collected from 77 patients at three representative sites, one healthy (probing depth [PD] £3 mm) and two diseased (PD ‡6 mm), at baseline and at 3 and 6 months after treatment. Patients received standard non-surgical periodontal treatment at each time point as appropriate. The outcome measure was improvement in probing depth of ‡2 mm. Concentrations of active enzymes (matrix metalloproteinase [MMP]-8, elastase, and sialidase) in GCF and subgingival plaque levels of Porphyromonas gingivalis, Tannerella forsythia, and Fusobacterium nucleatum were analyzed for prediction of the outcome measure.

    Results: Using threshold values of MMP-8 (94 ng/mL), elastase (33 ng/mL), sialidase (23 ng/mL), and levels of P. gingivalis (0.23%) and T. forsythia (0.35%), receiver operating characteristic curves analysis demonstrated that these biomarkers at baseline could differentiate healthy from diseased sites (sensitivity and specificity ‡77%). Furthermore, logistic regression showed that this combination of these biomarkers at baseline provided accurate predictions of treatment outcome (‡92%).

    Conclusion: The ‘‘fingerprint’’ of GCF enzymes and bacteria described here offers a way to predict the outcome of non-surgical periodontal treatment on a site-specific basis.

    J Periodontol 2017;88:1135-1144.