Structure of a complete mediator-RNA polymerase II pre-initiation complex

• Philip J. Robinson ^[1] ; Michael J. Trnka ^[2] ; David A. Bushnell ^[1]
  1. [1] Stanford University

     Stanford University

     Estados Unidos

     
  2. [2] University of California
• Localización: Cell, ISSN 0092-8674, Vol. 166, Nº. 6, 2016, págs. 1411-1422
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.