sRNA-mediated control of transcription termination in E.coli

• Nadezda Sedlyarova ^[1] ; Ilya Shamovsky ^[2] ; Binod K. Bharati ^[2]
  1. [1] University of Vienna

     University of Vienna

     Innere Stadt, Austria

     
  2. [2] New York University

     New York University

     Estados Unidos

     
• Localización: Cell, ISSN 0092-8674, Vol. 167, Nº. 1, 2016, págs. 111-121
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Bacterial small RNAs (sRNAs) have been implicated in various aspects of post-transcriptional gene regulation. Here, we demonstrate that sRNAs also act at the level of transcription termination. We use the rpoS gene, which encodes a general stress sigma factor σS, as a model system, and show that sRNAs DsrA, ArcZ, and RprA bind the rpoS 5′UTR to suppress premature Rho-dependent transcription termination, both in vitro and in vivo. sRNA-mediated antitermination markedly stimulates transcription of rpoS during the transition to the stationary phase of growth, thereby facilitating a rapid adjustment of bacteria to global metabolic changes. Next generation RNA sequencing and bioinformatic analysis indicate that Rho functions as a global “attenuator” of transcription, acting at the 5′UTR of hundreds of bacterial genes, and that its suppression by sRNAs is a widespread mode of bacterial gene regulation.