Epithelial-mesenchymal micro-niches govern stem cell lineage choices

• Hanseul Yang ; Rene C. Adam ; Yejing Ge ^[1]
  1. [1] Howard Hughes Medical Institute

     Howard Hughes Medical Institute

     Estados Unidos

     
• Localización: Cell, ISSN 0092-8674, Vol. 169, Nº. 3, 2017, págs. 483-496
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Adult tissue stem cells (SCs) reside in niches, which, through intercellular contacts and signaling, influence SC behavior. Once activated, SCs typically give rise to short-lived transit-amplifying cells (TACs), which then progress to differentiate into their lineages. Here, using single-cell RNA-seq, we unearth unexpected heterogeneity among SCs and TACs of hair follicles. We trace the roots of this heterogeneity to micro-niches along epithelial-mesenchymal interfaces, where progenitors display molecular signatures reflective of spatially distinct local signals and intercellular interactions. Using lineage tracing, temporal single-cell analyses, and chromatin landscaping, we show that SC plasticity becomes restricted in a sequentially and spatially choreographed program, culminating in seven spatially arranged unilineage progenitors within TACs of mature follicles. By compartmentalizing SCs into micro-niches, tissues gain precise control over morphogenesis and regeneration: some progenitors specify lineages immediately, whereas others retain potency, preserving self-renewing features established early while progressively restricting lineages as they experience dynamic changes in microenvironment.