Dynamic remodeling of membrane composition drives cell cycle through primary cilia excision

• Siew Cheng Phua ^[1] ; Shuhei Chiba ^[2] ; Masako Suzuki ^[3]
  1. [1] Johns Hopkins University

     Johns Hopkins University

     Estados Unidos

     
  2. [2] Osaka University

     Osaka University

     Kita Ku, Japón

     
  3. [3] Hamamutsu University

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• Localización: Cell, ISSN 0092-8674, Vol. 168, Nº. 1-2, 2017, págs. 264-279
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • The life cycle of a primary cilium begins in quiescence and ends prior to mitosis. In quiescent cells, the primary cilium insulates itself from contiguous dynamic membrane processes on the cell surface to function as a stable signaling apparatus. Here, we demonstrate that basal restriction of ciliary structure dynamics is established by the cilia-enriched phosphoinositide 5-phosphatase, Inpp5e. Growth induction displaces ciliary Inpp5e and accumulates phosphatidylinositol 4,5-bisphosphate in distal cilia. This change triggers otherwise-forbidden actin polymerization in primary cilia, which excises cilia tips in a process we call cilia decapitation. While cilia disassembly is traditionally thought to occur solely through resorption, we show that an acute loss of IFT-B through cilia decapitation precedes resorption. Finally, we propose that cilia decapitation induces mitogenic signaling and constitutes a molecular link between the cilia life cycle and cell-division cycle. This newly defined ciliary mechanism may find significance in cell proliferation control during normal development and cancer.