CRISPR-based technologies for the manipulation of eukaryotic genomes

• Alexis C. Komor ; Ahmed H. Badran ; David R. Liu ^[1]
  1. [1] Harvard University

     Harvard University

     City of Cambridge, Estados Unidos

     
• Localización: Cell, ISSN 0092-8674, Vol. 168, Nº. 1-2, 2017, págs. 20-36
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications. We describe recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems, and we highlight some of the remarkable advancements in basic research, biotechnology, and therapeutics science that these developments have facilitated.