Uncoupling of neurogenesis and differentiation during retinal development

• Peter Engerer ^[1] ; Sachihiro C Suzuki ^[2] ; Takeshi Yoshimatsu ^[2] ; Prisca Chapouton ^[4] ; Nancy Obeng ^[1] ; Benjamin Odermatt ^[3] ; Philip R Williams ^[5] ; Thomas Misgeld ^[6] ; Leanne Godinho ^[1]
  1. [1] Technical University Munich

     Technical University Munich

     Kreisfreie Stadt München, Alemania

     
  2. [2] University of Washington

     University of Washington

     Estados Unidos

     
  3. [3] University of Bonn

     University of Bonn

     Kreisfreie Stadt Bonn, Alemania

     
  4. [4] 3 Sensory Biology and Organogenesis German Research Center for Environmental Health Helmholtz Zentrum München Neuherberg Germany
  5. [5] 1 Institute of Neuronal Cell Biology Technische Universität München Munich Germany; 0Present address: Department of NeurologyF.M. Kirby Neurobiology CenterChildren's Hospital of BostonHarvard Medical SchoolBostonMAUSA
  6. [6] 1 Institute of Neuronal Cell Biology Technische Universität München Munich Germany; 5 Center of Integrated Protein Science (CIPSM) Munich Germany; 6 German Center for Neurodegenerative Diseases (DZNE) Munich Germany; 7 Munich Cluster of Systems Neurology (SyNergy) Munich Germany

  Mostrar afiliaciones +
• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 36, Nº. 9, 2017, págs. 1134-1146
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Conventionally, neuronal development is regarded to follow a stereotypic sequence of neurogenesis, migration, and differentiation. We demonstrate that this notion is not a general principle of neuronal development by documenting the timing of mitosis in relation to multiple differentiation events for bipolar cells (BCs) in the zebrafish retina using in vivo imaging. We found that BC progenitors undergo terminal neurogenic divisions while in markedly disparate stages of neuronal differentiation. Remarkably, the differentiation state of individual BC progenitors at mitosis is not arbitrary but matches the differentiation state of post‐mitotic BCs in their surround. By experimentally shifting the relative timing of progenitor division and differentiation, we provide evidence that neurogenesis and differentiation can occur independently of each other. We propose that the uncoupling of neurogenesis and differentiation could provide neurogenic programs with flexibility, while allowing for synchronous neuronal development within a continuously expanding cell pool.