Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

Dayahindara Veitía; Juan Liuzzi; Maira Avila; Zoraya de Guglielmo Cróquer; María Correnti de Plata

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Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

Veitía, Dayahindara ^[2] ; Liuzzi, Juan ^[1] ; Àvila, Maira ^[1] ; De Guglielmo, Zoraya ^[2] ; Correnti, María ^[3]
1. [1] Hospital Oncológico Andrés Grillasca
  
  Hospital Oncológico Andrés Grillasca
  
  Puerto Rico
2. [2] Laboratorio de Genética Molecular, Instituto de Oncología y Hematología-MPPS, Caracas.
3. [3] Instituto de Investigaciones Odontológicas Raúl Vincentelli, Facultad de Odontología de la UCV, Caracas.
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Localización: Journal of Oral Research, ISSN-e 0719-2479, ISSN 0719-2460, Vol. 6, Nº. 4, 2017, págs. 86-91
Idioma: inglés
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Resumen
- Introduction: Infection caused by potentially oncogenic viruses, such as HPV and EBV, favors the role of certain oncoproteins that can induce dysplasias and malignant lesions. Objective: To evaluate the prevalence of HPV and EBV and their relation with the expression of p53 and PCNA in patients with oral carcinoma. Methodology: Twenty-seven oral squamous cell carcinomas (OSCC) were evaluated; DNA extraction was conducted using the QIAamp DNA mini kit; viral detection was obtained using the INNO-LiPA kit for HPV, and nested PCR was used for EBV. The evaluation of molecular markers was performed through immunohistochemical staining. Results: The mean age of the patients was 60.55±13.94 years, and 52% of these were female. Of the patients, 59% were tobacco users and 63% were alcohol consumers. HPV was detected in 70% of the patients with the predominance of genotype 16 (60%). As for EBV infection, it was observed in 59% of cases. p53 and PCNA immunopositivity corresponded to 44% and 59%, respectively. The tongue was the anatomical location with highest positivity for both viruses as well as for the expression of molecular markers. The 48% of the cases presented infection by both viruses. Conclusion: HPV and EBV infection together with the expression of p53 and PCNA were more frequently observed in advanced stages of the disease, suggesting a more relevant role in the progression than in tumor genesis.