Tridimensional visualization and analysis of early human development
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[1] Institut de la Vision
Institut de la Vision
París, Francia
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- Localización: Cell, ISSN 0092-8674, Vol. 169, Nº. 1, 2017, págs. 161-173
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Generating a precise cellular and molecular cartography of the human embryo is essential to our understanding of the mechanisms of organogenesis in normal and pathological conditions. Here, we have combined whole-mount immunostaining, 3DISCO clearing, and light-sheet imaging to start building a 3D cellular map of the human development during the first trimester of gestation. We provide high-resolution 3D images of the developing peripheral nervous, muscular, vascular, cardiopulmonary, and urogenital systems. We found that the adult-like pattern of skin innervation is established before the end of the first trimester, showing important intra- and inter-individual variations in nerve branches. We also present evidence for a differential vascularization of the male and female genital tracts concomitant with sex determination. This work paves the way for a cellular and molecular reference atlas of human cells, which will be of paramount importance to understanding human development in health and disease.
