Utilizing Mechanistic Cross-Linking Technology To Study Protein–Protein Interactions: An Experiment Designed for an Undergraduate Biochemistry Lab

• Kara Finzel ^[2] ; Joris Beld ^[2] ; Michael D. Burkart ^[2] ; Louise K. Charkoudian ^[1]
  1. [1] Haverford College

     Haverford College

     City of Philadelphia, Estados Unidos

     
  2. [2] University of California-San Diego, United States
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 94, Nº 3, 2017, págs. 375-379
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Over the past decade, mechanistic cross-linking probes have been used to study protein–protein interactions in natural product biosynthetic pathways. This approach is highly interdisciplinary, combining elements of protein biochemistry, organic chemistry, and computational docking. Herein, we described the development of an experiment to engage undergraduate students in multidisciplinary research that leverages mechanistic cross-linking probes to study protein conformations and protein–protein interactions. This experiment provides students with a platform to learn chemoenzymatic synthesis, polyacrylamide gel electrophoresis, biochemical assays, and computational docking, all while exploring a contemporary biochemical topic.