Resumen de Analysis of grape rootstocks by microsatellite markers

János Májer, Gizella Jahnke, Péter Varga, Gitta Kocsisné Molnár, Erik Tarczal, László Kocsis

• The aim of this work was to determine the SSR profiles of 96 Vitis accessions (mainly rootstocks) for 19 loci, and to find genetic relatedness among them. Most molecular markers used in the last 30 years are not affected by the environment, giving a good tool for determination of genetic distances. Based on our earlier experience, SSR analysis was carried out on 96 grape accessions, mainly rootstocks. Analyses were carried out with 19 microsatellite loci. The loci were selected to cover all of the linkage groups to get a more precise impression of the genome of grape rootstocks. Results of SSR analyses and a dendrogram showing genetic relatedness are presented. Based on the results it can be established that most of the loci showed appropriate polymorphism. High similarity was detected between the clones of T5C and TK5BB. The Vitis sylvestris accessions showed close relatedness with the Vitis vinifera L. cultivars. Our results indicate a high polymorphism between grape rootstock accessions. Strong differences were detected between the so called ?clones? of T5C and TK5BB, suggesting, that they are not real clones, but different genotypes with highly similar morphological features.