Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq

• Autores: Daniel Gerlach, Christoph Stelzer
• Localización: Science, ISSN 0036-8075, Vol. 339, Nº 6123, 2013, págs. 1075-1077
• Idioma: inglés
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• Resumen

  • Genomic enhancers are important regulators of gene expression, but their identification is a challenge, and methods depend on indirect measures of activity. We developed a method termed STARR-seq to directly and quantitatively assess enhancer activity for millions of candidates from arbitrary sources of DNA, which enables screens across entire genomes. When applied to the Drosophila genome, STARR-seq identifies thousands of cell type–specific enhancers across a broad continuum of strengths, links differential gene expression to differences in enhancer activity, and creates a genome-wide quantitative enhancer map. This map reveals the highly complex regulation of transcription, with several independent enhancers for both developmental regulators and ubiquitously expressed genes. STARR-seq can be used to identify and quantify enhancer activity in other eukaryotes, including humans.