Enhanced accumulation of the isoprostane, 8-epi-PGF2a, in human aortic and pulmonary valves of patients with coronary heart disease.

• M.R. Mehrabi ^[1] ; N. Serbecic ^[1] ; C. Ekmekcioglu ^[1] ; F. Tamaddon ^[1] ; T. Wild ^[1] ; K. Plesch ^[1] ; H. Sinzinger ^[1] ; H.D. Glogar ^[1]
  1. [1] University of Vienna

     University of Vienna

     Innere Stadt, Austria

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 17, Nº. 4, 2002, págs. 1053-1059
• Idioma: inglés
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• Resumen

  • The aim of the present study was to investigate whether the isoprostane 8-epi-PGF2a differently accumulates in semilunar valves of patients suffering from coronary heart disease (CHD, n=19) as compared to valves from healthy heart donors (controls, n=6). Sections from isolated aortic and pulmonary valves were analyzed by semiquantitative immunohistochemistry. The 8-epi-PGF2a-content was determined by using a specific radioimmunoassay. The accumulation of 8-epi-PGF2a in both valves was higher in CHD-patients in comparison to controls (Aortic valves: 36.49±11.26% vs. 15.78±3.04%; pulmonary valves: 46.79±9.80% vs. 14.99±3.57%). The results from the radioimmunoassay revealed comparable findings in both groups (CHD vs. controls: 395.95±86.09 vs. 139.50±47.46 pg/mg protein in the aortic valves and 430.47±76.30 vs. 147.33±53.84 pg/mg protein in pulmonary valves). Pulmonary valves seem to be more susceptible to oxidative stress than aortic valves as evidenced by a higher accumulation of 8-epi-PGF2a in CHD patients. Considering the data presented in this study, we suggest that 8-epi-PGF2a is a valuable indicator of oxidative injury in human semilunar valves.