Enhanced expression of endothelin-1 and endothelin-converting enzyme-1 in acute hypoxic rat aorta

• Y. Doi ^[1] ; H. Kudo ^[1] ; T. Nishino ^[1] ; O. Yamamoto ^[1] ; T. Nagata ^[1] ; S. Nara ^[1] ; M. Morita ^[1] ; S. Fujimoto ^[1]
  1. [1] University of Occupational and Environmental Health Japan

     University of Occupational and Environmental Health Japan

     Kokurakita Ku, Japón

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 17, Nº. 1, 2002, págs. 97-105
• Idioma: inglés
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• Resumen

  • Deeply anesthetized male Wistar rats were perfused by Hanks' balanced salt solution bubbled with either 95%air and 5%CO2 (normoxic group) or 95%N2 and 5%CO2 (hypoxic group) from the thoracic aorta for 30 min, and the isolated abdominal aortae from both groups were used for electron microscopy, immunocytochemistry of endothelin (ET)-1 and ET-converting enzyme (ECE)-1, and in situ hybridization of preproET-1 mRNA. A remarkable increase in the number of Weibel-Palade (WP) bodies, storage sites of ET-1 and ECE-1, occurred in the hypoxic group when compared to the normoxic group. Immunoreactivities for ET-1 and ECE-1, and signals for preproET-1 mRNA were seen along the endothelia of both groups, but the intensities were significantly elevated in the hypoxic group. The increase in the number of ECE-1 immunoreactive gold particles was noticed especially in WP bodies in the hypoxic group. These findings indicate the enhancement of preproET-1 synthesis in the aortic endothelial cells as well as the acceleration of ET-1 processing in increased WP bodies in such cells in an experimentally hypoxic condition of the rat aortae.