Molecular markers to detect genetic variation in superior clones of grapevine (vitis vinifera l. 'keshmeshi')

• Autores: Hamed Doulati Baneh
• Localización: XI International Conference on Grapevine Breeding and Genetics / coord. por Shao-Hua Li, Zong-Ming Cheng, Douglas D. Archbold, J. London, 2015, ISBN 9789462610767, págs. 183-188
• Idioma: inglés
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• Resumen

  • To assess the genetic differences among clones of grapevine (Vitis vinifera L.) ?Keshmeshi?, ten selected clones from a clonal selection program were analyzed by 23 Simple Sequence Repeats (SSR) and 7 AFLP primer combinations. No intra-varietal differences could be detected by SSRs among clones whereas 8 out of the 499 AFLP fragments generated by the seven primer combinations were polymorphic. The number of markers ranged from 44 (E34-M34) to 97 (E31-M32) with an average of 71.3 fragments per primer combination. Cluster analysis based on AFLP data separate all the clones of ?Keshmeshi? into two groups. The first group includes nine white berry skin clones, without any genetic differences and the second group has only a red berry skin clone. AFLP analyses could only detect the red berry clone of ?Keshmeshi? from the other white berry clones.