Variations of B cell subpopulations in peripheral blood of healthy Mexican population according to age: Relevance for diagnosis of primary immunodeficiencies
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L. Berrón-Ruíz [3] ; G. López-Herrera [4] ; C.E. Ávalos-Martínez [1] ; C. Valenzuela-Ponce [3] ; E. Ramírez-SanJuan [1] ; G. Santoyo-Sánchez [2] ; F. Mújica Guzmán [4] ; F.J. Espinosa-Rosales [4] ; L. Santos-Argumedo [3]
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[1] Instituto Politécnico Nacional
Instituto Politécnico Nacional
México
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[2] Universidad Nacional Autónoma de México
Universidad Nacional Autónoma de México
México
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[3] Centro de Investigación y de Estudios Avanzados-IPN, México
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[4] Instituto Nacional de Pediatría-SSa, México
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- Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 44, Nº. 6, 2016, págs. 571-579
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Background Peripheral blood B cells include lymphocytes at various stages of differentiation, each with a specific function in the immune response. All these stages show variations in percentage and absolute number throughout human life. The numbers and proportions of B subpopulation are influenced by factors such as gender, age, ethnicity, and lifestyle. This study establishes reference values according to age of peripheral blood B cell subtypes in healthy Mexican population.
Methods Peripheral blood from healthy new-borns and adults were analysed for total B cell subpopulations, using surface markers such as CD19, IgM, IgD, CD21, CD24, CD27, and CD38, to identify naïve, memory with and without isotype switch, double-negative, transitional, and plasmablast cells.
Results We observed a significant variation in terms of frequency and absolute counts between all groups analysed. Values from each B cell subpopulation show variations according to age.
Conclusions In order to attempt to elucidate reference values for B cell subpopulation, the present study evaluated a population sample of healthy blood donors from this region. Values reported here can also be used as a tool for diagnosis of diseases in which B cell maturation is affected.
