Molecular force spectroscopy with a DNA origami–based nanoscopic force clamp

• Autores: Bettina Wünsch, Phil Holzmeister, Wooli Bae
• Localización: Science, ISSN 0036-8075, Vol. 354, Nº 6310, 2016, págs. 305-307
• Idioma: inglés
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• Resumen

  • Forces in biological systems are typically investigated at the single-molecule level with atomic force microscopy or optical and magnetic tweezers, but these techniques suffer from limited data throughput and their requirement for a physical connection to the macroscopic world. We introduce a self-assembled nanoscopic force clamp built from DNA that operates autonomously and allows massive parallelization. Single-stranded DNA sections of an origami structure acted as entropic springs and exerted controlled tension in the low piconewton range on a molecular system, whose conformational transitions were monitored by single-molecule Förster resonance energy transfer. We used the conformer switching of a Holliday junction as a benchmark and studied the TATA-binding protein–induced bending of a DNA duplex under tension. The observed suppression of bending above 10 piconewtons provides further evidence of mechanosensitivity in gene regulation.