Cutaneous (tPA) and Skeletal (TnI) mRNA as Markers of Aging in Contused Wound

• Samah F. Ibrahim ^[1] ; Marwa Issak ^[1] ; Amany A. Bayoumy ^[1] ; Dina S. Abd El-Fatah ^[1]
  1. [1] Cairo University

     Cairo University

     Egipto

     
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 61, Nº. 4, 2016, págs. 1007-1010
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Wound age estimation is one of the most important forensic aspects. Troponin I (TnI) and many cytokines, for example, tissue plasminogen activator (tPA), are involved in wound inflammation and healing. Skeletal (TnI) and cutaneous (tPA) mRNA was detected using real-time PCR in 25 female albino rats. They were divided into 5 groups: control and 4 injured groups. Injured groups were sacrificed 1, 6, 24, and 30 h after inflicting contused wound. The expression levels of cutaneous (tPA) were decreased significantly at 1, 6, and 30 h after contusion (71.7%, 30.7 and 16.9%), while the expression levels of skeletal (TnI) were increased significantly at 1 and 6 h post-traumatic, then they gradually decreased until reaching normal levels at 24 h and assumed significantly lower levels at 30 h postcontusion. These results suggested that the determination of cutaneous (tPA) and skeletal (TnI) mRNA levels was useful for wound age estimation.