Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production

• Amin S.M. Salehi ^[1] ; Conner C. Earl ^[1] ; Christina Muhlestein ^[1] ; Bradley C. Bundy ^[1]
  1. [1] Brigham Young University

     Brigham Young University

     Estados Unidos

     
• Localización: International journal of developmental biology, ISSN 0214-6282, Vol. 60, Nº. Extra 7-9, 2016 (Ejemplar dedicado a: Special Issue: Cell-free Extracts in Development & Cancer Research / coord. por Jacek Z. Kubiak), págs. 237-243
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Cell-free protein synthesis has been around for decades but it has never been close to becoming a robust tool for the production of biotherapeutic agents. In this review, we focus on how Escherichia coli-based cell-free protein synthesis can be modified in various ways to produce challenging, complex anticancer biotherapeutics. Here we report progress in extract preparation and its relation to cell-free cancer research. The future prospects of cell-free technology and its potential in various areas of cancer therapeutics production are also highlighted.