Membrane fission by dynamin: what we know and what we need to know

• Bruno Antonny ^[13] ; Christopher Burd ^[1] ; Pietro De Camilli ^[14] ; Elizabeth Chen ^[2] ; Oliver Daumke ^[15] ; Katja Faelber ^[15] ; Marijn Ford ^[3] ; Vadim A Frolov ^[4] ; Adam Frost ^[16] ; Jenny E Hinshaw ^[5] ; Tom Kirchhausen ^[6] ; Michael M Kozlov ^[7] ; Martin Lenz ^[8] ; Harry H Low ^[9] ; Harvey McMahon ^[10] ; Christien Merrifield ^[17] ; Thomas D Pollard ^[1] ; Phillip J Robinson ^[11] ; Aurélien Roux ^[12] ; Sandra Schmid ^[2]
  1. [1] Yale University

     Yale University

     Town of New Haven, Estados Unidos

     
  2. [2] Southwestern Medical Center

     Southwestern Medical Center

     Estados Unidos

     
  3. [3] University of Pittsburgh

     University of Pittsburgh

     City of Pittsburgh, Estados Unidos

     
  4. [4] Universidad del País Vasco/Euskal Herriko Unibertsitatea

     Universidad del País Vasco/Euskal Herriko Unibertsitatea

     Leioa, España

     
  5. [5] National Institute of Diabetes and Digestive and Kidney Diseases

     National Institute of Diabetes and Digestive and Kidney Diseases

     Estados Unidos

     
  6. [6] Boston Children's Hospital

     Boston Children's Hospital

     City of Boston, Estados Unidos

     
  7. [7] Tel Aviv University

     Tel Aviv University

     Israel

     
  8. [8] University of Paris-Saclay

     University of Paris-Saclay

     Arrondissement de Palaiseau, Francia

     
  9. [9] Imperial College London

     Imperial College London

     Reino Unido

     
  10. [10] MRC Laboratory of Molecular Biology

      MRC Laboratory of Molecular Biology

      Cambridge District, Reino Unido

      
  11. [11] University of Sydney

      University of Sydney

      Australia

      
  12. [12] Université de Genève

      Université de Genève

      Genève, Suiza

      
  13. [13] 1 CNRS Institut de Pharmacologie Moléculaire et Cellulaire Université de Nice Sophia‐Antipolis Valbonne France
  14. [14] 3 Departments of Neuroscience and Cell Biology Howard Hughes Medical Institute and Kavli Institute for Neuroscience Yale University School of Medicine New Haven CT USA
  15. [15] 5 Department of Crystallography Max‐Delbrück Centrum für Molekulare Medizin Berlin Germany
  16. [16] 9 Department of Biochemistry and Biophysics University of California, San Francisco San Francisco CA USA
  17. [17] 17 Institute for Integrative Biology of the Cell Gif sur Yvette Cedex France

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• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 35, Nº. 21, 2016, págs. 2270-2284
• Idioma: inglés
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• Resumen

  • The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.