High Resolution Melting (HRM) analysis on "VviDXS" to reveal muscats or non-muscats among autochthonous Greek wine producing grape varieties

• Autores: Georgios Merkouropoulos, Ioannis Ganopoulos, Andreas Doulis, Nikolaos Nikolaou, Photini Mylona
• Localización: OENO ONE: Journal international des sciences de la vigne et du vin = International journal of vine and wine sciences, ISSN 1151-0285, Vol. 50, Nº 3, 2016, págs. 161-167
• Idioma: inglés
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• Resumen

  • Muscat flavor in grapes is associated with a substitution of a Single Nucleotide Polymorphism (SNP) located at position 1822 (SNP1822G>T) within the coding sequence of the VviDXS gene. Various methods, including the use of High Resolution Melting (HRM) analysis, have been suggested to discriminate different SNP allelic states including the molecular discrimination of the muscat from the non-muscat grape varieties, thus providing the ability to minimize lengthy grape breeding programs when selecting for grape muscat flavor before the fruit maturity stage. HRM analysis on the SNP1822 was performed on a group of 128 wine producing grape varieties in order to separate the muscat from the non-muscat genotypes before they are used for further breeding activities. This approach could be used either as a single-step prescreening method or in accordance with recently published methodology to elucidate on varietal characterization and authentication as these are important requirements concerning nurseries, growers and winemakers.