RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity

• Gen Shiratsuchi ^[2] ; Katsuyoshi Takaoka ^[1] ; Tomoko Ashikawa ^[2] ; Hiroshi Hamada ^[1] ; Daiju Kitagawa ^[2]
  1. [1] Osaka University

     Osaka University

     Kita Ku, Japón

     
  2. [2] 1 Centrosome Biology Laboratory, Center for Frontier Research, National Institute of Genetics Mishima, Shizuoka, Japan
• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 34, Nº. 1, 2014, págs. 97-114
• Idioma: inglés
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• Resumen

  • Formation of a new centriole adjacent to a pre-existing centriole occurs only once per cell cycle. Despite being crucial for genome integrity, the mechanisms controlling centriole biogenesis remain elusive. Here, we identify RBM14 as a novel suppressor of assembly of centriolar protein complexes. Depletion of RBM14 in human cells induces ectopic formation of centriolar protein complexes through function of the STIL/CPAP complex. Intriguingly, the formation of such structures seems not to require the cartwheel structure that normally acts as a scaffold for centriole formation, whereas they can retain pericentriolar material and microtubule nucleation activity. Moreover, we find that, upon RBM14 depletion, a part of the ectopic centriolar protein complexes in turn assemble into structures more akin to centrioles, presumably by incorporating HsSAS-6, a cartwheel component, and cause multipolar spindle formation. We further demonstrate that such structures assemble in the cytoplasm even in the presence of pre-existing centrioles. This study sheds light on the possibility that ectopic formation of aberrant structures related to centrioles may contribute to genome instability and tumorigenesis.