Proteomics of a fuzzy organelle: interphase chromatin

• Georg Kustatscher ^[1] ; Nadia Hégarat ^[2] ; Karen L H Wills ^[1] ; Cristina Furlan ^[1] ; Jimi-Carlo Bukowski-Wills ^[1] ; Helfrid Hochegger ^[2] ; Juri Rappsilber ^[1]
  1. [1] Wellcome Trust Centre for Cell Biology

     Wellcome Trust Centre for Cell Biology

     Reino Unido

     
  2. [2] University of Sussex

     University of Sussex

     Reino Unido

     
• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 33, Nº. 6, 2014, págs. 648-664
• Idioma: inglés
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• Resumen

  • Chromatin proteins mediate replication, regulate expression, and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle, it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead, we propose a new approach that provides a quantitative assessment of a protein's probability to function in chromatin. We integrate chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including 1840 previously uncharacterized proteins. We demonstrate the power of our large-scale data-driven annotation during the analysis of cyclin-dependent kinase (CDK) regulation in chromatin. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology.