Factor-independent transcription pausing caused by recognition of the RNA–DNA hybrid sequence

• Aleksandra Bochkareva ^[1] ; Yulia Yuzenkova ^[1] ; Vasisht R Tadigotla ^[2] ; Nikolay Zenkin ^[1]
  1. [1] Newcastle University

     Newcastle University

     Reino Unido

     
  2. [2] Boston University

     Boston University

     City of Boston, Estados Unidos

     
• Localización: EMBO journal: European Molecular Biology Organization, ISSN 0261-4189, Vol. 31, Nº. 3, 2011, págs. 630-639
• Idioma: inglés
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• Resumen

  • RNA polymerase pausing during transcription is implicated in controlling gene expression. This study identifies a new type of pausing mechanism, by which the RNAP core recognizes the shape of base pairs of the RNA–DNA hybrid, which determines the rate of translocation and the nucleotide addition cycle. The expression of a number of viral and bacterial genes is shown to be subject to this mechanism.