Collagen barrier membranes may accelerate bacterial growth in vitro: A potential clinical risk to regenerative procedures

• Autores: Shimshon Slutzkey, Shlomo Matalon, Avital Kozlovsky, Zvi Artzi
• Localización: Quintessence International, ISSN-e 0033-6572, Vol. 46, Nº. 1, 2015, págs. 43-50
• Idioma: inglés
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• Resumen

  • Objectives: Evaluate the antibacterial properties of three commercial collagen membranes for guided bone regeneration (GBR).

    Method and Materials: BioGide®, OsseoGuard®, and CopiOs® membranes were tested for antibacterial properties against Streptococcus sanguinis, Staphylococcus aureus, and Staphylococcus epidermidis using a direct contact test (DCT). Samples of each membrane, sized 5 × 2 mm, were fixed to the side wall of 6 wells in a 96-well microtiter plate. Bacterial suspension was placed on each sample. The plate was incubated at 37°C for 1 hour to allow suspension fluid evaporation. Fresh medium was added to each well and the plate was mixed. Non-contaminated membrane samples served as negative control. Bacterial growth was monitored spectrophotometrically at 650 nm for 24 hours. ANOVA and Tukey's comparison tests were used for the statistical analysis.

    Results: The presence of the membrane samples did not disrupt bacterial growth. However, the presence of OsseoGuard membrane accelerated bacterial growth rate of S sanguinis and S epidermidis as compared to the other two membranes and control samples (P < .001), and of S aureus as compared to the control alone (P < .001).

    Conclusions: The tested collagen membranes have no antibacterial properties. However, OsseoGuard presents a significant bacterial growth enhancement effect with possible significant clinical implications on the success of regenerative procedures around teeth and implants.