UV-Visible First-Derivative Spectrophotometry Applied to an Analysis of a Vitamin Mixture

• F. Aberásturi ^[1] ; A. I. Jiménez ^[1] ; F. Jiménez ^[1] ; J. J. Arias ^[1]
  1. [1] Universidad de La Laguna

     Universidad de La Laguna

     San Cristóbal de La Laguna, España

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 78, Nº 6, 2001, pág. 793
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • A simple new experiment that uses UV-vis spectrophotometry to introduce undergraduate chemistry students to multicomponent analysis is presented and a method for the simultaneous determination of three vitamins using derivative spectrophotometry (zero-crossing method) is described. The methodology is simple and easy to apply and allows the determination of folic acid, pyridoxine, and thiamine over the concentration ranges 1.02-14.28, 1.00-16.00, and 6.00-20.00 μg mL-1, respectively. The resulting errors were nearly always less than 5%.