Simultaneous PCR detection of ica cluster and methicillin and mupirocin resistance genes in catheter-isolated Staphylococcus

• Juana V. Martín-López; Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, Spain; and Cell Biology and Microbiology Department, University of La Laguna, Santa Cruz de Tenerife, Spain ; Oscar Díez-Gil ^[1] ; Manuel Morales ^[1] ; Ninivé Batista ^[1] ; Jesús Villar ^[1] ; Félix Claverie-Martín ^[1] ; Sebastián Méndez-Álvarez; Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria University Hospital, Santa Cruz de Tenerife, Spain; and Cell Biology and Microbiology Department, University of La Laguna, Santa Cruz de Tenerife, Spain
  1. [1] Hospital Clinico Universitario de Valencia

     Hospital Clinico Universitario de Valencia

     Valencia, España

     
• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 7, Nº. 1, 2004, págs. 63-66
• Idioma: inglés
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• Resumen

  • Recent data show that more than 50% of catheter-associated bloodstream infections are caused by staphylococci. Staphylococcal infections produced by intercellular-adhesion cluster (ica) carriers can be even more problematic due to the presence of methicillin and mupirocin resistance genes. In the present study, a multiplex PCR protocol that allows the simultaneous identification of staphylococci and detection of both the ica and methicillin and/or mupirocin resistance genes was developed. Furthermore, the method allows differential detection of the ica locus from Staphylococcus aureus and Staphylococcus epidermidis. [Int Microbiol 2004; 7(1):63–66]