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Conjugative plasmid mediated inducible nickel resistance in Hafnia alvei 5-5

    1. [1] Department of Biology, Research Institute for Basic Science, Kyunghee University, Seoul, Korea
    2. [2] Institut für Mikrobiologie, Georg-August-Universität, Göttingen, Germany
  • Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 6, Nº. 1, 2003, págs. 57-64
  • Idioma: inglés
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  • Resumen
    • Hafnia alvei 5-5, isolated from a soil-litter mixture underneath the canopy of the nickel-hyperaccumulating tree Sebertia acuminata (Sapotaceae) in New Caledonia, was found to be resistant to 30 mM Ni2+ or 2 mM Co2+. The 70-kb plasmid, pEJH 501, was transferred by conjugation to Escherichia coli, Serratia marcescens, and Klebsiella oxytoca. Transconjugant strains expressed inducible nickel resistance to between 5 and 17 mM Ni2+, and cobalt resistance to 2 mM Co2+. A 4.8-kb Sal–EcoRI fragment containing the nickel resistance determinant was subcloned, and the hybrid plasmid was found to confer a moderate level of resistance to nickel (7 mM Ni2+) even to E. coli. The expression of nickel resistance was inducible by exposure to nickel chloride at a concentration as low as 0.5 mM Ni2+. By random TnphoA´-1 insertion mutagenesis, the fragment was shown to have structural genes as well as regulatory regions for nickel resistance. Southern hybridization studies showed that the nickel-resistance determinant from pEJH501 of H. alvei 5-5 was homologous to that of pTOM9 from Alcaligenes xylosoxydans 31A.


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