Regional metabolite concentrations in the brain of healthy dogs measured by use of short echo time, single voxel proton magnetic resonance spectroscopy at 3.0 Tesla

• Inés ^[1] ; Henning ^[2] ; Dieter ^[3] ; Patrick ^[4] ; Matthias ^[5]
  1. [1] Carrera
  2. [2] Richter
  3. [3] Meier
  4. [4] R. Kircher
  5. [5] Dennler

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• Localización: American Journal of Veterinary Research, ISSN-e 1943-5681, ISSN 0002-9645, Vol. 76, Nº. 2, 2015, págs. 129-141
• Idioma: inglés
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  • Abstract American Journal of Veterinary Research February 2015, Vol. 76, No. 2, Pages 129-141 doi: 10.2460/ajvr.76.2.129 Regional metabolite concentrations in the brain of healthy dogs measured by use of short echo time, single voxel proton magnetic resonance spectroscopy at 3.0 Tesla Inés Carrera DVM; Henning Richter DVM; Dieter Meier PhD; Patrick R. Kircher PhD; Matthias Dennler DVM Clinic of Diagnostic Imaging, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland. (Carrera, Richter, Kircher, Dennler); Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland. (Carrera); MR Zentrum USZ, Institute for Biomedical Engineering, University and ETH (Swiss Federal Institute of Technology), Zurich, 8092 Zurich, Switzerland. (Meier) Address correspondence to Dr. Carrera (icarrera@vetclinics.uzh.ch).

    OBJECTIVE To investigate regional differences of relative metabolite concentrations in the brain of healthy dogs with short echo time, single voxel proton magnetic resonance spectroscopy (1H MRS) at 3.0 T.

    ANIMALS 10 Beagles.

    PROCEDURES Short echo time, single voxel 1H MRS was performed at the level of the right and left basal ganglia, right and left thalamus, right and left parietal lobes, occipital lobe, and cerebellum. Data were analyzed with an automated fitting method (linear combination model). Metabolite concentrations relative to water content were obtained, including N-acetyl aspartate, total choline, creatine, myoinositol, the sum of glutamine and glutamate (glutamine-glutamate complex), and glutathione. Metabolite ratios with creatine as the reference metabolite were calculated. Concentration differences between right and left hemispheres and sexes were evaluated with a Wilcoxon signed rank test and among various regions of the brain with an independent t test and 1-way ANOVA.

    RESULTS No significant differences were detected between sexes and right and left hemispheres. All metabolites, except the glutamine-glutamate complex and glutathione, had regional concentrations that differed significantly. The creatine concentration was highest in the basal ganglia and cerebellum and lowest in the parietal lobes. The N-acetyl aspartate concentration was highest in the parietal lobes and lowest in the cerebellum. Total choline concentration was highest in the basal ganglia and lowest in the occipital lobe.

    CONCLUSIONS AND CLINICAL RELEVANCE Metabolite concentrations differed among brain parenchymal regions in healthy dogs. This study may provide reference values for clinical and research studies involving 1H MRS performed at 3.0 T.