Monitoring of Levamisole Concentration in Serum of Traffic Participants after Cocaine Consumption
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[1] Hannover Medical School (MHH) Institute of Legal Medicine Hannover German
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[2] Hannover Medical School (MHH) Institute of Legal Medicine Hannover Germany
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- Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 61, Nº. Extra 1, 2016, págs. 250-251
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
This study highlights the problem of levamisole-adulterated cocaine in context of active traffic participation. For the purposes of levamisole concentration monitoring in human serum, an analytical method based on LC-MS/MS and solid-phase extraction was applied. A Luna 5 lm C18 (2) 100 A, 150 mm 9 2 mm column and a mobile phase consisting of A (H2O/methanol = 95/5, v/v) and B (H2O/methanol = 3/97, v/v), both with 10 mM ammonium acetate and with 0.1% acetic acid (pH = 3.2), were used. The validation experiments demonstrated that the method applied was appropriate for levamisole quantification in human serum. For 23% of levamisole-positive samples, the concentrations exceeded 20 ng/mL. Therefore, the interaction of this drug with cocaine has to be considered as important for active traffic participation. As a consequence, monitoring of levamisole concentration in human serum is recommended, as long as it is used as cocaine adulterant.
