Temperature-Dependent Postmortem Changes in Human Cardiac Troponin-T (cTnT):: An Approach in Estimation of Time Since Death
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Sachil Kumar [1] ; Wahid Ali [1] ; Uma S. Singh [1] ; Ashutosh Kumar [1] ; Sandeep Bhattacharya [1] ; Anoop K. Verma [1] ; Raja Rupani [1]
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[1] King George's Medical University
King George's Medical University
India
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- Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 61, Nº. Extra 1, 2016, págs. 241-245
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Estimation of time of death is an indispensible requirement of every medico-legal autopsy, but unfortunatel y, there is not a single method by which it could be determined accurately. This study focused on the temperature-dependent postmortem degrad ation of cardiac troponin-T and its association with postmortem interval (PMI) in human. The analysis involved extraction of the protein, separation by denaturing gel electrophoresis (SDS-PAGE), and visualization by Western blot using cTnT-specific monoclonal antibodies. The area of the bands within a lane was quantified by scanning and digitizing the image using Gel Doc (Universal Hood). The results indicate a characteristic banding pattern among human cadavers (n = 6) and a pseudo-linear relationship between percentage of cTnT degradation and the log of the time since death (r > 0.95), which can be used to estimate the postmortem interval. The data presented demonstrate that this technique can provide an extended time range during which PMI can be more accurately estimated.
