Yes-associated protein (YAP) expression is involved in epithelial–mesenchymal transition in hepatocellular carcinoma
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S. Wang [1] ; H. Li [1] ; G. Wang [1] ; T. Zhang [1] ; B. Fu [1] ; M. Ma [2] ; Z. Quan [2] ; G. Chen [1]
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[1] Third Affiliated Hospital of Sun Yat-sen University
Third Affiliated Hospital of Sun Yat-sen University
China
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[2] Shanghai Jiaotong University, China
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- Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 18, Nº. 2 (February 2016), 2016, págs. 172-177
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Purpose To investigate biological impact of the downregulation of yes-associated protein (YAP) through RNA interference in the process of epithelial–mesenchymal transition in MHCC97H and MHCC97L.
Methods MHCC97H and MHCC97L cells were transiently transfected by YAP-siRNA. Furthermore, protein expressions and mRNA levels of characteristic markers of epithelial–mesenchymal transition (E-cadherin, N-cadherin) were examined by Western blotting and real-time polymerase chain reaction, and transwell invasion assay was used to detect changes of invasiveness of MHCC97H and MHCC97L cells.
Results The transfected group with YAP-siRNA in MHCC97H after 72 h by Western blotting showed obviously higher expression of E-cadherin compared with the control group (P < 0.05), and lower expression of N-cadherin (P < 0.05). In MHCC97L cells, the expression of E-cadherin was also significantly increased (P < 0.05); however, N-cadherin expression did not significantly change (P > 0.05). Moreover, compared with the control group, Transwell invasion assay showed that the number of the transfected groups was significantly decreased in MHCC97H and MHCC97L cell lines (both P < 0.05). The result of real-time polymerase chain reaction indicated that mRNA levels of E-cadherin increased (P < 0.05), but the mRNA levels of N-cadherin did not significantly change (P > 0.05) in these two cell lines, indicating some effects of post-transcriptional regulation mechanism after silencing YAP.
Conclusions YAP expression in human hepatocellular carcinoma cell lines MHCC97H and MHCC97L is closely related with the characteristic markers of epithelial–mesenchymal transition, N-cadherin and E-cadherin expression.
