Physical Profile and Impact of a Calcium-Incorporated Implant Surface on Preosteoblastic Cell Morphologic and Differentiation Parameters: ¡A Comparative Analysis

• Marco ^[1] ; Luca ^[2] ; Cristina ^[9] ; Giuseppe ^[3] ; Emanuele ^[4] ; Domenica ^[5] ; Maddalena ^[6] ; Agnese ^[7] ; Alberto ^[8]
  1. [1] Lollobrigida
  2. [2] Lamazza
  3. [3] Formisano
  4. [4] Serra
  5. [5] Laurito
  6. [6] Romanelli
  7. [7] ¡Molinari¡
  8. [8] De Biase
  9. [9] Capuano

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• Localización: The International Journal of Oral & Maxillofacial Implants, ISSN-e 0882-2786, Vol. 31, Nº. 1, 2016, págs. 223-231
• Idioma: inglés
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  • Purpose: To assess and compare topographic features and preosteoblastic cell responses of a new hydrothermally treated, calcium-incorporated surface against other commercially available implant surfaces. Materials and Methods: Four different surfaces were the subject of comparison in this study: machined (MC), resorbable blast media (RBM), sandblasted/large-grit/acid-etched (SLA), and calcium-incorporated SLA (Ca-SLA). Surface morphology and roughness were first characterized by scanning electron microscope (SEM) and white light interferometer, respectively. Preosteoblastic MC3T3-E1 cells were then cultured on the titanium surfaces. Cell morphology was observed at 24 hours, 48 hours, 7 days, and 15 days by SEM; differentiation was assessed at 7, 11, and 15 days by assaying alkaline phosphatase (ALP) activity and osteocalcin (OCN) levels. Results: Surface characterization revealed nanotopographic features on Ca-SLA. At topographic analysis, SLA and Ca-SLA showed similar roughness values. Significant differences in cell differentiation parameters were found only at 15 days between the SLA surfaces (both Ca-incorporated and nonincorporated) and MC. Conclusion: Collectively, this study demonstrated that hydrothermal treatment determines the formation of nanotopography without altering the SLA microtopography. Moreover, Ca-SLA and SLA induce MC3T3-E1 cell differentiation at comparable levels.