Resumen de The Binding Constant for Complexation of Bilirubin to Bovine Serum Albumin. An Experiment for the Biophysical Chemistry Laboratory
Kathryn R. Williams, Bhavin Adhyaru, Russell E. Pierce, Stephen G. Schulman
Students use fluorescence spectroscopy to measure the binding constant for bilirubin-bovine serum albumin complexation by the Method of Continuous Variations. The experiment applies fundamental concepts of physical chemistry to a commercially available system of biological interest. The experiment is easily completed in a single laboratory period, and students obtain binding constants on the order of 10 7, in good agreement with literature values.
