Fatty Acid Profiles for Differentiating Growth Medium Formulations Used to Culture Bacillus cereus T-strain Spores

• Christopher J. Ehrhardt ^[1] ; Devonie L. Murphy ^[2] ; James M. Robertson ^[3] ; Jason D. Bannan ^[4]
  1. [1] Virginia Commonwealth University

     Virginia Commonwealth University

     Estados Unidos

     
  2. [2] Federal Bureau of Investigation, Laboratory Division Counterterrorism and Forensic Science Research Unit Visiting Scientist Program Quantico VA 22135
  3. [3] Federal Bureau of Investigation, Laboratory Division Counterterrorism and Forensic Science Research Unit Quantico VA 22135
  4. [4] Federal Bureau of Investigation, Laboratory Division Biological Program Science Advisor Quantico VA 22135

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• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 60, Nº. 4, 2015, págs. 1022-1029
• Idioma: inglés
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• Resumen

  • Microbial biomarkers that indicate aspects of an organism’s growth conditions are important targets of forensic research. In this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. Bacillus cereus T-strain spores were grown in medium formulations supplemented with one of the following: peptone (meat protein), tryptone (casein protein), soy protein, and brain–heart infusion. Cellular biomass was profiled with fatty acid methyl ester (FAME) analysis. Results showed peptone cultures produced spores enriched in straight-chained lipids. Tryptone cultures produced spores enriched in branched-odd lipids when compared with peptone, soy, and brain–heart formulations. The observed FAME variation was used to construct a set of discriminant functions that could help identify the nutrients in a culturing recipe for an unknown spore sample. Blinded classification tests were most successful for spores grown on media containing peptone and tryptone, showing 88% and 100% correct identification, respectively