Comparison of Four Saliva Detection Methods to Identify Expectorated Blood Spatter

• Hee-Yeon Park ^[1] ; Bu-Nam Son ^[2] ; Young-Il Seo ^[3] ; Si-Keun Lim ^[4]
  1. [1] Busan Institute of National Forensic Service DNA Analysis Section Gyeongsangnam‐do South Korea
  2. [2] Gyongnam Provincial Police Agency Scientific Investigation Center Gyeongsangnam‐do South Korea
  3. [3] National Forensic Service Forensic Safety Division Gangwon‐do South Korea
  4. [4] National Forensic Service Forensic DNA Division Gangwon‐do South Korea

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• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 60, Nº. 6, 2015, págs. 1571-1576
• Idioma: inglés
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• Resumen

  • Blood spatter analysis is an important step for crime scene reconstruction. The presence of saliva in blood spatter could indicate expectorated blood which is difficult to distinguish from impact spatter. In this study, four saliva test methods (SALIgAE â , Phadebas â sheet, RSID TM -Saliva kit, and starch gel diffusion) were compared to identify the best method for detecting expectorated blood spatter. The RSIDtm -Saliva kit showed the highest sensitivity even when saliva was mixed with blood, and was not inhibited by the presence of blood. The SALIgAE â test provided easy and rapid results, but the yellow color of a positive reaction was overwhelmed by the red color of the blood. The starch gel diffusion method and the Phadebas â sheet exhibited relatively low sensitivity and the assay took a long time. When using the RSIDtm -Saliva kit for identifying saliva in blood, results should be read within 10 min.