Effect of topical application of melatonin on serum levels of C-reactive protein (CRP), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in patients with type 1 or type 2 diabetes and periodontal disease

• Antonio Cutando ^[1] ; Javier Montero ^[2] ; Rafael Gómez-de Diego ^[3] ; María-José Ferrera ; Antonio Lopez-Valverde ^[2]
  1. [1] Universidad de Granada

     Universidad de Granada

     Granada, España

     
  2. [2] Universidad de Salamanca

     Universidad de Salamanca

     Salamanca, España

     
  3. [3] University of Alfonso X, Madrid,

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• Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 7, Nº. 5 (diciembre), 2015, págs. 628-633
• Idioma: inglés
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• Dialnet Métricas: 4 Citas
• Resumen

  • Background: The present clinical trial study was designed to assess the effect of topical application of melatonin on serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and C-reactive protein (CRP) in patients with diabetes and periodontal disease in comparison with healthy controls.

    Material and Methods: Serum levels of TNF-α and IL-6 were measured by enzyme-linked immunosorbent assay and CRP by nephelometry by using the proper commercial kits in 30 patients with diabetes and periodontal disease, and also in a control group of 30 healthy subjects. Periodontograms were performed using the Florida Probe®.

    Patients with diabetes were treated with a topical application of melatonin (1% orabase cream formula) once daily for 20 days. Healthy subjects were treated with a placebo orabase cream.

    Results: Patients with diabetes and periodontal disease had significantly higher mean levels of serum TNF-α, IL-6 and CRP than healthy subjects ( P < 0.001). Following topical melatonin application, there was a statistically signi - ficant decrease in the gingival index and pocket depth ( P < 0.001) as well as a significant decrease in IL-6 and CRP serum levels ( P < 0.001). Local melatonin application in patients with diabetes and periodontal disease resulted in a significant decrease in CRP and IL-6 serum levels as well as an improvement in the gingival index and pocket depth. Patients with periodontal disease had significantly higher serum CRP, IL-6 and TNF-α values by comparison with healthy subjects.

    Conclusions: We conclude that melatonin can modulate the inflammatory action of these molecules in periodontal patients