Nested PCR for detection of HSV-1 in oral mucosa

• Miranda-Masoumeh Jalouli ^[1] ; Jamshid Jalouli ^[1] ; Bengt Hasséus ^[2] ; Jenny Öhman ^[2] ; Jan-Michaél Hirsch ^[1] ; Lars Sand ^[1]
  1. [1] Uppsala University

     Uppsala University

     Uppsala domkyrkoförs., Suecia

     
  2. [2] University of Gothenburg

     University of Gothenburg

     Suecia

     
• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 20, Nº. 6 (Noviembre), 2015
• Idioma: inglés
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• Resumen

  • Background: It has been estimated that 15%-20% of human tumours are driven by infection and inflammation, and viral infections play an important role in malignant transformation. The evidence that herpes simplex virus type 1 (HSV-1) could be involved in the aetiology of oral cancer varies from weak to persuasive.

    This study aimed to investigate by nested PCR (NPCR) the prevalence of HSV-1 in samples from normal oral mucosa, oral leukoplakia, and oral squamous cell carcinoma (OSCC).

    Material and Methods: We investigated the prevalence of HSV-1 in biopsies obtained from 26 fresh, normal oral mucosa from healthy volunteers as well as 53 oral leukoplakia and 27 OSCC paraffin-embedded samples.

    DNA was extracted from the specimens and investigated for the presence of HSV-1 by nested polymerase chain reaction (NPCR) and DNA sequencing.

    Results: HSV-1 was detected in 14 (54%) of the healthy samples, in 19 (36%) of the oral leukoplakia samples, and in 14 (52%) of the OSCC samples. The differences were not statistically significant.

    Conclusions: We observed a high incidence of HSV-1 in healthy oral mucosa, oral leukoplakia, and OSCC tissues.

    Thus, no connection between OSCC development and presence of HSV-1 was detected.