Equilibrium Gel Filtration Chromatography for the Measurement of Protein-Ligand Binding in the Undergraduate Biochemistry Laboratory

• Douglas B. Craig ^[1]
  1. [1] University of Winnipeg

     University of Winnipeg

     Canadá

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 82, Nº 1, 2005, pág. 96
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Equilibrium gel filtration liquid chromatography is a technique used for the quantitation of ligand binding. We have designed an experiment for the senior undergraduate laboratory using this technique for the measurement of the binding of ATP to cytochrome c. This experiment introduces the student to the use of liquid chromatography and absorbance spectrometry and permits a quantitative result within a three-hour period. The experiment complements classroom discussions regarding liquid chromatography, spectroscopy, dynamic equilibrium, and kinetic parameters.