Applicability of the ParaDNA â Screening System to Seminal Samples

• Nicholas D. Tribble ^[2] ; Jamie A.D. Miller ^[1] ; Nick Dawnay ^[3] ; Nicola J. Duxbury ^[3]
  1. [1] King's College London

     King's College London

     Reino Unido

     
  2. [2] LGC Forensics Culham Science Centre Abingdon Oxfordshire U.
  3. [3] LGC Forensics Culham Science Centre Abingdon Oxfordshire U.K

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• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 60, Nº. 3, 2015, págs. 690-692
• Idioma: inglés
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• Resumen

  • Seminal fluid represents a common biological material recovered from sexual assault crime scenes. Such samples can be prescreened using different techniques to determine cell type and relative amount before submitting for full STR profiling. The ParaDNA r Screening System is a novel forensic test which identifies the presence of DNA through amplification and detection of two common STR loci (D16S539 and TH01) and the Amelogenin marker. The detection of the Y allele in samples could provide a useful tool in the triage and sub- mission of sexual assault samples by enforcement authorities. Male template material was detected on a range of common sexual assault evidence items including cotton pillow cases, condoms, swab heads and glass surfaces and shows a detection limit of 1 in 1000 dilution of neat semen. These data indicate this technology has the potential to be a useful tool for the detection of male donor DNA in sexual assault casework.