Electrostatic Potential Maps and Natural Bond Orbital Analysis: Visualization and Conceptualization of Reactivity in Sanger’s Reagent
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Jeffery D. Mottishaw [1] ; Adam R. Erck [1] ; Jordan H. Kramer [1] ; Haoran Sun [1] ; Miles Koppang [1]
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[1] University of South Dakota
University of South Dakota
City of Vermillion, Estados Unidos
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 92, Nº 11, 2015, págs. 1846-1852
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Frederick Sanger’s early work on protein sequencing through the use of colorimetric labeling combined with liquid chromatography involves an important nucleophilic aromatic substitution (SNAr) reaction in which the N-terminus of a protein is tagged with Sanger’s reagent. Understanding the inherent differences between this SNAr reaction and other nucleophilic substitution reactions (SN1 and SN2) can be challenging for students learning organic chemistry. Here, both electrostatic potential (ESP) maps and natural bond orbital (NBO) analyses are employed to visualize and conceptualize Sanger’s key observation of the difference in reactivity between 2,4-dinitrochlorobenzene and 2,4-dinitrofluorobenzene. The utility of this method is extended to compare the reactivity of a series of halobenzenes for SNAr fluorination, a widely used reaction in pharmaceutical and medicinal fields. In combination with experimental results from the literature, the ESP maps and NBO analyses are consistent with and provide excellent corroboration with the reactivity of different substrates toward SNAr reactions.
