Evaluation of Degradation in DNA from Males with a Quantitative Gender Typing, Endpoint PCR Multiplex

• Byron C. Smith ^[2] ; Emily Vandegrift ^[3] ; Valerie Mattimore Fuller ^[4] ; Robert W. Allen ^[1] ; School of Forensic Sciences
  1. [1] Oklahoma State University

     Oklahoma State University

     Estados Unidos

     
  2. [2] Tulsa Police Department Forensic Laboratory Tulsa OK
  3. [3] Tennessee Department of Public Safety Nashville TN
  4. [4] Ministry of Legal Affairs Government of Saint Lucia National Forensic Science Laboratory Tapion Castries West Indies

  Mostrar afiliaciones +
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 60, Nº. 2, 2015, págs. 399-408
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Evidentiary samples submitted to a forensic DNA laboratory occasionally yield DNA that is degraded. Samples of intact chromosomal DNA (both nuclear and mitochondrial) were subjected to a heating protocol to induce DNA degradation. The DNAs were then analyzed using a multiplex PCR assay that amplifies targets of low and high molecular weight on the X/Y and mitochondrial chromosomes. If degradation is random, the amplification of larger DNA targets should be more adversely affected by degradation than smaller targets. In nuclear and mitochondrial DNA from a male donor, exhibiting degradation, DNA quantity estimates based upon higher molecular weight amplicons (HMW) are significantly lower than estimates made using low molecular weight (LMW) Q-TAT amplicons. DNA degradation esti- mated using this approach correlated well with actual fluorescence associated with HMW and LMW STR alleles amplified from the same genomic DNA templates. Q-TAT is thus useful not only as a quantitation tool, but also as an indicator of template degradation.