A Silver-Stain Modification of Standard Histological Slide Preparation for Use in Anthropology Analyses

• Deborrah C. Pinto ^[1] ; Eric D. Pace ^[2]
  1. [1] Harris County Institute of Forensic Sciences Houston TX
  2. [2] MLD Pathology Houston TX
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 60, Nº. 2, 2015, págs. 391-398
• Idioma: inglés
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• Resumen

  • The traditional histology method typically employed by forensic anthropologists involves plastic embedding of undecalcified bone. The embedded sample is then cut by a diamond blade saw and ground to the required thickness of ~50–100 microns using a grinder.

    There are several limitations to this method: Cement lines may be blurred; depth-of-field artifacts may result from viewing thick sections; and medicolegal offices with limited budgets may not be able to invest in additional equipment or training for this method. A silver nitrate stain modification of the standard histology preparation technique of decalcified bone is presented. The benefits of this technique are that: Cement lines are viewed clearly; no depth-of-field artifacts are present; and because this is a modification of the standard technique used by histology laboratories typically employed by medicolegal offices, no additional equipment or training is required