Chemistry and Flatulence:: An Introductory Enzyme Experiment

• John R. Hardee ^[1] ; Tina M. Montgomery ^[1] ; Wray H. Jones ^[1]
  1. [1] Henderson State University

     Henderson State University

     Township of Caddo, Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 77, Nº 4 (April), 2000, págs. 498-498
• Idioma: inglés
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• Resumen

  • An inexpensive introductory-level enzyme experiment was developed using raffinose family sugars extracted from green split peas as a substrate and the enzymes alpha-galactosidase and sucrase found in Beano. The reaction studied was the hydrolysis of raffinose family sugars to galactose, glucose, and fructose, and the reaction rate was determined using a retail glucometer to measure the concentration of glucose. Results are given on the effect of substrate concentration, enzyme concentration, temperature, and heavy metals on enzyme activity.