Examining the Rate of Renaturation for Genomic DNA Isolated from E. coli:

• Nayhan Fancy ^[1] ; Andrew F. Mehl ^[1]
  1. [1] Knox College

     Knox College

     Township of Galesburg City, Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 76, Nº 5 (May), 1999, págs. 646-646
• Idioma: inglés
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• Resumen

  • The second-order rate constant for the renaturation of DNA is determined for E. coli genomic DNA. The procedure involves the initial melting of the double-stranded DNA at high temperature and then an abrupt lowering of the temperature so that renaturation can occur. The absorbance at 260 nm is monitored during the entire procedure.