Cristina Cordeiro, Rafael Seoane Prado, Ana Camba, Elena Lendoiro Belío, María Sol Rodríguez Calvo, Duarte Nuno Pessoa Vieira, José Ignacio Muñoz Barús
Research into maximizing the speed, precision, and reliability of estimating the postmortem interval (PMI) has been a recurring object of investigation and methodologies based on the vitreous humor (VH) have provided good results. However, contamination from causes not readily apparent, such as blood, can occur, and thus lead not only to an erroneous estimation of PMI, but also interfere with the correct identification of other substances in the VH. We have developed a flow cytometry method which quantifies blood contamination and is able to detect erythrocytes in 1:750,000 dilution of contaminated VH which affects the results of hypoxanthine. It is an improvement on the previous more complex mass spectrometry method, being faster, more sensitive, and readily available. As such, it could be proposed for therapid screening of appropriate samples by detecting and eliminating blood contaminated samples from PMI estimation
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