Capillary Electrophoresis:: Part I. Theoretical and Experimental Background

• Christine L. Copper ^[1]
  1. [1] United States Naval Academy

     United States Naval Academy

     Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 75, Nº 3 (March), 1998, págs. 343-347
• Idioma: inglés
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• Resumen

  • Separation is the cornerstone of many areas of chemistry. Quite often, the goal of a chemical experiment is to isolate and analyze a particular compound. Capillary electrophoresis (CE is emerging as a powerful separation tool.

    Separation in CE is based on differences in solute mobilities when a strong electric field is applied across a separation buffer solution. Compared to conventional liquid chromatographic methods, CE offers advantages of high efficiency, short analysis time, simple apparatus, small sample and separation buffer volumes, and ease in changing the separation buffer. Additionally, selectivity in CE separations can be easily altered through the use of different buffer additives.