Resumen de HPLC:: Early and Recent Perspectives
In this article, we provide personal reflections on the early days of HPLC and recent developments. HPLC has emerged from a technique that could only be imagined in the early 1960's to sales over $1.4 billion in 1994. Key to the advances has been the translation of theory to practice, in the ability to pack small particle diameter matrices of 5 mm to yield high efficiency columns. Chemically bonded phases have become a major separation mode in HPLC, particularly in the reversed phase mode. We note that the ruggedness of such columns are orders of magnitude better today than when they were introduced almost 25 years ago. The contributions of many people to this success is noted. We next turn to a brief look at the role of biochemists and analytical chemists in the development of liquid phase separation methods of biological substances. In the early years, the two communities did not strongly interact; however, today their connection in the field of biopolymer separations is quite significant. Since the future holds even more overlap, the need for analytical chemistry students to know fundamentals in the biological sciences is great.
