Rapid Microscale Isolation and Purification of Yeast Alcohol Dehydrogenase Using Cibacron Blue Affinity Chromatography

• Autores: Chad Morgan, Neil Moir
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 73, Nº 11 (November), 1996, págs. 1040-1041
• Idioma: inglés
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• Resumen

  • A rapid microscale procedure has been developed for the isolation and purification of yeast alcohol dehydrogenase. Glass beads are used for cytolysis, PEG precipitation for partial purification and a cibacron blue affinity column for the final step. A 27.5 fold purification can be achieved in 2 - 3 hours.