MicroRNA-15a down-regulation is associated with adverse prognosis in human glioma
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T. Xie [1] ; P. Liu [1] ; L. Chen [1] ; Z. Chen [1] ; Y. Luo [1] ; X. Chen [1] ; Y. Feng [1] ; X. Luo [1]
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[1] The First People’s Hospital of Jingmen, China
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- Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 17, Nº. 7 (July), 2015, págs. 504-510
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Objective The aim of this study was to investigate whether miR-15a has prognostic relevance in human gliomas.
Methods The expression levels of miR-15a were analyzed in glioma surgical resection tissues by microarray and quantitative real-time PCR. Survival analysis by the Kaplan–Meier method was performed to assess prognostic significance.
Results Downregulation of miR-15a was detected in most primary gliomas, which was confirmed by qRT-PCR analysis. Additionally, the down-regulation of miR-15a was significantly associated with the WHO grade (P = 0.003), the low KPS (P = 0.027), time to recurrence (P = 0.044) and the poor OS (P = 0.046). Using Kaplan–Meier analysis, a comparison of survival curves of low versus high expresser of miR-15a revealed a highly significant difference in OS (P = 0.001) and DFS (P = 0.006), which suggested that low expression of miR-15a is associated with a worse prognosis. Multivariate analyses showed that miR-15a expression was independent risk factors predicting OS [Hazard ratio (HR), 7.52; 95 % confidence interval (CI), 2.63–21.47; P = 0.002] and DFS [HR, 11.56; 95 % CI, 5.17–25.96; P < 0.001] in glioma.
Conclusions These findings indicated for the first time that the expression of miR-15a is significantly correlated with prognosis in glioma patients, suggesting that the miR-15a may serve as independent prognostic marker.
